Allergy Lateral Flow Assay

Allergy Lateral Flow Assay  is a rapid test for the determination of allergen specific Immunoglobulin E (sIgE) in human serum, plasma or whole blood

BACKROUND

The worldwide frequency of allergies increased significantly over the past decades. The term allergy is often used for type I hypersensitivity reactions (immediate type reactions), whose symptoms generally occur within 30-60 min after contact with the allergen. The most frequent symptoms are: hay fever (rhinitis), conjunctivitis, hives (urticaria), allergic asthma and as the most dangerous manifestation anaphylaxis (the anaphylactic shock). The allergens causing type I hypersensitivity reactions are mostly proteins derived from the natural environment e.g. plant pollen, animal hair, food, mites and insect venoms. The characteristic of type I allergies is the involvement of allergen specific immuno-globulins (antibodies) of class E (sIgE). Hence, the detection of sIgE is an important tool of modern allergy diagnostics.

Allergy Lateral Flow Assay

Allergy Lateral Flow Assay  is a screening test, which enables the user to perform an allergy test very fast and reliable. By choosing an appropriate allergen solutions a symptom based allergy diagnosis is possible. The test result are available in 20 minutes and are stated, when read with the LFA Reader, in Units/ml and classes according to the RAST classes. Allergy Lateral Flow Assay consist of one basis unit – the Allergy Lateral Flow Assay  Basis Set – in combination with different, freely selectable allergen solutions, as single or mixed allergens (Screens) or as recombinant or native, highly purified allergens. Allergy Lateral Flow Assay Basis Set is available as single- (REF 1800010) and eight-strip cassette (REF 184000) – plus variable allergen solutions. (Download: Flyer )

PRINCIPLE

To perform the test the patient´s sample is transferred to the sample application point of the Basis Set. Immediately afterwards, the desired allergen solution is applied. During incubation of 20 min the liquid is driven through the device by capillary flow. The allergen specific IgE of the sample binds specifically to its corresponding allergens of the allergen solution. The labelled allergens are retained at the test line (T) by a capture molecule. At the same time, the sIgE bound to the allergen is bound by an antibody coupled to colored particles (conjugate). The intensity of the color reaction at the test line is proportional to the amount of immune complexes consisting of ligand tagged allergens, sIgE and IgE specific conjugate. The signal intensity ranges from faintly pink (low titer of sIgE) to dark ruby (high titer of sIgE). Access conjugate, which is not bound at the test line, forms a dark ruby control line (C) during incubation. (Download: Instruction for use Allergy Lateral Flow Assay)


AVAILABE ALLERGENS

More than 150 freely selectable allergen solutions, as single or mixed allergens (Screens) or as recombinant or native, highly purified allergens are available for Allergy Lateral Flow Assay.  All available allergens can be found in the current List of Allergy Lateral Flow Assay  allergens and the current list of recombinant or native, highly purified allergens (Download: Flyer List Allergy Lateral Flow Assay Allergens, Flyer recombinante Allergene)

SYSTEMS

On the one hand the evaluation of Allergy Lateral Flow Assay can be done macroscopic, in which the intensity of the test line is proportional to the amount of bound sIgEs. The signal intensity ranges from faintly pink (low titer of sIgE) to dark ruby (high titer of sIgE). On the other hand Allergy Lateral Flow Assay can be evaluated with the help of the Lateral Flow Assay  Reader. The LFA Reader is a mobile measuring device for the quantitative evaluation and documentation of Allergy Lateral Flow Assay. The test result is stated in Units (U/mL) and classes, according to the RAST classes. For documentation the results can be printed, stored on an internal memory of the Lateral Flow Assay  Reader or exported. (Download: Flyer LFA Reader)

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AlyssaGan
Phone: (416) 613-9131 
Email: info@alyssagan.ca

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